| Data | In casa | Ospite | Risultato |
|---|---|---|---|
| 14/09/25 01.30 | New England Revolution | Toronto FC | 1:1 |
| 14/09/25 01.30 | CF Montreal | St. Louis City SC | 0:2 |
| 14/09/25 01.30 | Cincinnati FC | Nashville SC | 2:1 |
| 14/09/25 01.30 | D.C. United | Orlando City | 1:1 |
| 14/09/25 01.30 | Charlotte FC | Inter Miami CF | 3:0 |
| 14/09/25 01.30 | Atlanta United FC | Columbus Crew | 4:5 |
| 14/09/25 02.40 | FC Dallas | Austin FC | 2:0 |
| 14/09/25 02.40 | Seattle Sounders | Los Angeles Galaxy | 2:2 |
| 14/09/25 02.40 | San Jose Earthquakes | Los Angeles FC | 2:4 |
| 14/09/25 02.40 | Chicago Fire | New York City FC | 1:3 |
| 14/09/25 03.30 | Real Salt Lake | Sporting Kansas City | 2:1 |
| 14/09/25 03.30 | Colorado Rapids | Houston Dynamo | 2:1 |
| 14/09/25 03.35 | Vancouver Whitecaps | Philadelphia Union | 7:0 |
| 14/09/25 04.30 | San Diego FC | Minnesota United FC | 1:3 |
| 14/09/25 04.30 | Portland Timbers | New York Red Bulls | 2:1 |
| 17/09/25 01.30 | Inter Miami CF | Seattle Sounders | -:- |